Special Specimen Collection and Handling

QuantiFERON®-TB Gold In Tube

Collect: 1 mL whole blood in each of three Cellestis QuantiFERON®-TB Gold In Tube collection tubes (Nil, TB, and Mitogen tubes: ARUP Supply #45112).

QuantiFERON-TB Gold In Tube Instructional Videos   
     
Blood Collection Tubes (mixing method and storage before Incubation)

Transport: Original collection tubes, after incubation and centrifugation, at 2–8°C.
Pediatric Collect/Transport: Original collection tubes, after incubation and centrifugation, at 2–8°C.
Remarks: Immediately following collection, each specimen tube must be mixed vigorously by shaking the tube up and down 10 times to ensure that the entire inner surface of the tube has been coated with blood. After mixing, incubate upright at 37°C for 16–24 hours (within 16 hours of collection). After incubation, centrifuge for 15 minutes at 2000–3000 RCF (g).
Unacceptable Conditions: Specimens that have not been centrifuged; ambient specimens.
Stability: After incubation and centrifugation: ambient: two hours; refrigerated: four weeks; frozen: four months.

 

Related Information

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org