ARUP's Laboratory Test Directory

T-Cell Clonality Assessment by Restriction Fragment-Southern Blot Hybridization : 0055596

Mnemonic: TCRFSBHF

Methodology: Restriction Fragment Southern Blot Hybridization
Performed: DNA isolation: Sun-Sat; Assay: Varies
Reported: 7-14 days
Specimen Required: Collect: Lavender (EDTA) or bone marrow (EDTA) OR fresh tissue.

Specimen Preparation: : Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue.

Storage/Transport Temperature: Whole blood OR Bone marrow: Refrigerated. OR Fresh tissue: Frozen on dry ice.

Unacceptable Conditions: Serum. Frozen whole blood or bone marrow. Clotted or grossly hemolyzed specimens. Paraffin-embedded tissue.

Stability (collection to initiation of testing): Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable Fresh tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year

Interpretive Data: By report

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: A positive result indicates the presence of monoclonal T-cell population within the sample.  A negative result does not entirely exclude the presence of a T-cell receptor gene rearrangement (or monoclonal T-cell population) in the sample.
CPT Code(s): 83891 Isolation; 83892 x3 Digestion; 83894 Gel Electrophoresis; 83897 Southern transfer; 83896 Nucleic acid probe; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
Cross References: T-Cell Gene Rearrangement (T-Cell Clonality Assessment by Restriction Fragment-Southern Blot Hybridization, Fluid)
 
 

 

 

 
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