ARUP's Laboratory Test Directory

Antimicrobial Susceptibility - AFB/Mycobacterium tuberculosis Primary Panel : 0060347

Mnemonic: MA MTBPRIM

Methodology: Broth Macrodilution
Performed: Sun-Sat
Reported: 21-28 days
Specimen Required: Collect: Actively growing isolate of Mycobacterium tuberculosis in pure culture.

Specimen Preparation: Transport sealed container with pure culture on solid or liquid media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature: Room temperature.
Submit specimen according to Infectious Substance, Category A, shipping guidelines.

Submit specimen according to Infectious Substance, Category A, shipping guidelines.

Unacceptable Conditions: Mixed cultures or non-viable organisms. Organisms submitted on agar plate.

Stability (collection to initiation of testing): Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: The interpretation provided is based on results for the following drugs at the stated concentrations:
Drugs tested: Ethambutol: 5.0 µg/mL, isoniazid: 0.1 µg/mL (0.4 µg/mL if resistant to 0.1 µg/mL), pyrazinamide: 100 µg/mL, and rifampin: 1.0 µg/mL.

This procedure screens isolates of M. tuberculosis complex for drug resistance. The procedure does not use serial dilutions to provide quantitative MIC values. Single critical concentrations for each antimycobacterial agent used have been defined by the United States Public Health Service.
CPT Code(s): 87188 x4
 
 

 

 

 
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