ARUP's Laboratory Test Directory

Galactose-1-Phosphate in Red Blood Cells : 0081296

Mnemonic: GAL1P RBC

Methodology: Gas Chromatography-Mass Spectrometry
Performed: Tue
Reported: 2-9 days
Specimen Required: Collect: Lavender (EDTA) or green (sodium or lithium heparin).

Specimen Preparation: DO NOT FREEZE. Place tube on wet ice immediately after collection. Transport 5 mL whole blood. (Min: 2 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Frozen or room temperature specimens.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
 
Test Number Components Reference Interval
 Galactose-1-phosphate 0.0-1.0 mg/dL
 Galactose-1-phosphate (ug/g Hb) 0-53 ug/g Hb
 Galactose-1-phosphate (umol/g Hb) 0.00-0.20 umol/g Hb

Note: Alternate acceptable specimen is frozen, washed red blood cells.
1. Centrifuge whole blood immediately for 10 minutes at 800 g RCF.
2. Discard the plasma and buffy coat layers.
3. Add cold 0.9 percent saline solution to the red cells (about 2 times the volume of cells) and mix gently by inverting the tube.
4. Centrifuge for 10 minutes at 800 g RCF.
5. Aspirate and discard the supernatant.
6. Repeat the wash (steps 3 through 5) 2 more times.
7. After the 3rd wash and centrifugation, remove the supernatant and a thin layer of the top cells. 
8. Transfer washed cells to an ARUP standard transport tube freeze. Ship on dry ice and indicate on sample and/or requisition that washed red cells have been submitted.
 
Washed red cells must be frozen.  Ambient and refrigerated washed cells are unacceptable
CPT Code(s): 84378
Cross References: Gal1P (Galactose-1-Phosphate in Red Blood Cells), Galactose 1 (Galactose-1-Phosphate in Red Blood Cells), Galactose 1 Phosphate (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Analyte (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Metabolite(s) (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Monitoring (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Therapy (Galactose-1-Phosphate in Red Blood Cells), GALT Analyte (Galactose-1-Phosphate in Red Blood Cells), GALT Monitoring (Galactose-1-Phosphate in Red Blood Cells)
 
 

 

 

 
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