ARUP's Laboratory Test Directory

Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot : 2001769

Mnemonic: BORDM

Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, heat-inactivated, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval:
Test Number Components Reference Interval
Bordetella pertussis Antibody, IgM by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgM antibody.
1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 U/mL or greater: Positive - IgM antibody to Bordetella pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by Immunoblot Negative

Interpretive Data: Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.

Refer to Statement D under Testing Information at http://www.aruplab.com.
Note: If Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
CPT Code(s): 86615 Bordetella IgM; if reflexed add 86615 for Bordetella IgM Immunoblot
Cross References: Pertussis Antibody (Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot) , Whooping Cough (Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot)
 
 

 

 

 
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