ARUP's Laboratory Test Directory

KRAS Mutation Detection with Reflex to BRAF Codon 600 Mutation Detection : 2001932

Mnemonic: KRAS RFLX

Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Sun-Sat
Reported: 10-14 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides). Protect paraffin block from excessive heat..

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Remarks: Include surgical pathology report.

Unacceptable Conditions: Frozen specimens. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Less than 50 percent tumor in tissue. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Interpretive Data: Refer to report.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: This assay detects mutations in codons 12, 13, and 61. If KRAS is not detected, BRAF codon 600 Mutation Detection will be performed. Additional charges apply.
CPT Code(s): 88381 Microdissection; 83907 Lysis; 83898 x2 Amplification; 83904 x2 Sequencing; 83912 Interpretation and report, if reflexed, add 83898 Amplification; 83904 Sequencing; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test oncologic or inherited disorders.
Cross References: Cetuximab (Erbitux) (KRAS Mutation Detection with Reflex to BRAF Codon 600 Mutation Detection) , Panitumumab (Vectibix) (KRAS Mutation Detection with Reflex to BRAF Codon 600 Mutation Detection)
 
 

 

 

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org